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Professor of Research of Biology and Biomedical Engineering
Director of the W.M. Keck Center for Cellular Imaging
M.S., Physics, Annamalai University, Tamil Nadu, India, 1974
M.S./Ph.D. , Biomedical Engineering, Indian Institute of Technology, Madras,
1980/1983
ap3t@virginia.edu
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Research Interests
Our current research interest is energy-based FLIM-FRET microscopy and spectroscopy,
determining when and where proteins associate with one another in living cells
and tissues. We have developed different light microscopy imaging systems to
investigate the cellular signaling of calcium, pH, and protein-protein interactions,
as well as for use as diagnostic systems in clinical imaging. These systems
include digitized video microscopy, digital deconvolution, confocal, multi-photon
excitation, fluorescence lifetime (FLIM), time-resolved, anisotropy, and fluorescence
resonance energy transfer (FRET) microscopy.
FRET is a process by which a radiationless transfer of energy occurs from a
fluorophore in the excited state to an acceptor molecule in close proximity.
The range over which this resonance energy transfer can occur is limited to
about 0.01 micrometers, and the efficiency of energy transfer is extraordinarily
sensitive to the distance between fluorophores. The measurement of FRET in the
microscope provides a noninvasive approach to visualize the spatiotemporal dynamics
of interactions between protein partners labeled with green fluorescent proteins
in the living cell.
Selected Publications
A. Periasamy, P. Wodnicki, X. F. Wang, S. Kwon, G. W. Gordon, and B. Herman.
Time resolved fluorescence lifetime imaging microscopy using picosecond pulsed
tunable dye laser system. Review of Scientific Instruments, 67:3722-3731, 1996.
A. Periasamy and R. Day. Visualizing protein interactions in living cells using
digitized GFP imaging and FRET microscopy. Methods in Cell Biology, 58:293-314,
1998.
A. Perisamy, P. Skoglund, C. Noakes, and R. Keller. An evaluation of two-photon
excitation versus confocal and digital deconvolution fluorescence microscopy
imaging in Xenopus morphogenesis. Microscopy Research & Technique, 47:172-181,
1999.
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