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Lawrence Lab Protocols

We have posted frequently used and published protocols here. Hopefully these will facilitate collaboration and help with training students interested in working in the Lawrence Lab.

*Disclaimer* Be sure to take appropriate safety precautions and seek appropriate training before using any equipment these protocols require. These are guidelines and we make no claim as to their effectiveness without personal training.

Protocol	Category	Title
WI0001	Microcontact Printing	Preparation of Sigma-Coted Wells for Microcontact Inking
WI0002	Blood Isolation	Preparation of Neutrophils from Whole Blood for Selectin Adhesion Assay
WI0003	Surface Preparation	Preparation of Piranha-Washed Glass Slides
WI0004	Cell Culture	Preparation of RPMI Culture Media
WI0005	Surface Preparation	Preparation of Micropatterned Slides Using Fluid Wicking
WI0006	Microparticle Preparation	Preparation of PrIgG Polystyrene Beads
WI0007	Microscopy	Preparation of Bead-Coated Surface for PSF Determination
WI0008	Microscopy	Operation of the Nikon Eclipse TE300
WI0009	Surface Preparation	Preparation of Polystyrene Slides with Uniform Protein Adsorption
WI0010	Surface Preparation	Preparation of Glass Slides for Protein Adsorption
WI0011	Surface Preparation	Preparation of Amine-Functionalized Glass Slides
WI0012	Site Density Determination	Determination of Microbead Site Density by Flow Cytometry
WI0013	Cell Culture	Culture and Splitting of Anchorage-Dependent Cells
WI0014	Cell Culture	Preparation of Cells for Liquid Nitrogen Storage
WI0015	Photolithography	Transfer of Photolithographic Mask Pattern from Transparency to Glass
WI0016	Photolithography	Photolithography with SU-8 2035 Photoresist
WI0017	Genetic Engineering	Linearization of Supercoiled DNA for the Stable Transfection of Cell Lines
WI0018	Flow Cytometry	Antibody Staining for Surface Antigens and Fixation of Cells for Flow Cytometry
WI0019	Mycoplasma Testing	Cell Line Mycoplasma Contamination Detection with PCR
WI0020		Number Reserved Until Publication
WI0021		Number Reserved Until Publication
WI0022	Genetic Engineering	Stable Transfection of Cell Lines by Electroporation